Measurement

Part:BBa_K2944006:Design

Designed by: Davindra Singh   Group: iGEM19_Concordia-Montreal   (2019-10-16)


pTDH3-mVenus-tPGK1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Assembly of all three parts is done with the type IIs enzyme BsaI, so restriction sites of this enzyme must not be found elsewhere than flanking the individual parts prior to assembly.


Source

The promoter and terminator are found in Saccharomyces cerevisiae whereas the fluorescent protein mVenus is derived from the jellyfish Aequorea victoria.

References